IVF, also known as in vitro fertilization embryo transfer, is an assisted reproductive technology used to help infertile couples achieve their reproductive dreams.The following will elaborate on the operation steps of IVF from four aspects: preparation, egg collection and sperm treatment, embryo culture and embryo transfer.
preparation
In order to ensure the success rate of IVF transplantation, medical personnel need to carry out a series of examinations on patients before surgery, including uterine morphology examination, tubal patency examination and male semen examination.In addition, doctors also need to assess the couple's age, physical condition, reproductive hormone levels, ovulation cycle and other related factors to determine the best treatment plan.Before surgery, female patients need to measure the endometrial thickness to determine the best time for embryo implantation.In addition, couples also need professional psychological guidance to understand and accept the risks and possible complications of IVF transplantation.
Egg collection and sperm treatment
When the female patient's ovary develops to the best state, the doctor will perform the follicle puncture operation with technical difficulty, and extract the follicle from the ovary through a slender needle.The eggs will be sent to the laboratory for sperm treatment.At the same time, male patients will be asked to provide fresh semen samples.Samples will be processed in the laboratory to obtain high-quality sperm.In general, semen samples will be purified and screened by multi-layer centrifugation and density gradient centrifugation to ensure the availability of fertile sperm.
Embryo culture
After the egg and sperm combine, a fertilized egg is formed.These fertilized eggs will be transferred into a special culture dish and placed in a constant temperature and humidity incubator for culture.At the same time, doctors will choose the appropriate embryo culture time, usually 2-6 days, according to the condition of patients and the technical level of the laboratory.During this process, doctors will pay close attention to the development of embryos, evaluate the quality of embryos and select the best embryos for transplantation.
embryo transfer
Embryo transfer is the last step of IVF transfer.When the embryo develops to its best state, the doctor will choose an appropriate time to transfer the embryo to the uterus of the female patient.This procedure is usually performed through the vagina without anesthesia.Before the operation, the doctor will use vaginal ultrasound to confirm the status of the endometrium and determine the best location for embryo implantation.During the operation, doctors will use professional instruments to carefully transplant the embryos into the endometrium.Usually, one or more embryos are transferred to improve the success rate of pregnancy.After the operation, female patients need to take proper rest and recovery until the pregnancy results.To sum up, the operation steps of IVF include preparation, egg collection and sperm treatment, embryo culture and embryo transfer.This process needs professional doctors to operate and cooperate with patients.The gradual progress of IVF transplantation technology provides a path for couples who cannot conceive naturally to realize their reproductive dreams.
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