How to obtain sperm for IVF
In vitro fertilization (IVF) is a kind of assisted reproductive technology, which can help couples who are difficult to conceive naturally realize their reproductive dreams. Among them, obtaining sperm is one of the most important steps in IVF. This article will elaborate on how to obtain sperm from IVF in four aspects: donor selection, sperm collection, sperm storage and preliminary follow-up treatment.
Selection of sperm donors
Choosing suitable donors is the key to ensure the quality of IVF. Usually, hospitals will strictly screen donors to ensure their health and eliminate genetic diseases. Donors usually need to be 20-45 years old, have no history of hereditary diseases, and undergo strict physical examination and STD screening. At the same time, the donor's genes will also be matched with those of couples who need IVF treatment to improve the success rate of pregnancy.
In addition, some couples may prefer to choose their relatives or relatives as donors. In this case, it is necessary to ensure that donors also meet the selection criteria of hospitals, and carry out the same matching procedures to ensure safety and success rate.
Sperm collection
Sperm collection is usually carried out by manual or electronic ejaculation. Manual ejaculation refers to the direct collection of discharged semen by manually stimulating the donor's reproductive organs. Electronic ejaculation is to use an electronic device to stimulate the donor to ejaculate naturally by simulating natural stimulation.
During the collection process, doctors will wear appropriate disinfection gloves and use disinfection equipment to ensure a sterile environment during the operation. Donors usually need to collect semen through a prepared special container in a precision controlled preparation room, and try to avoid external pollution.
sperm storage
Once the semen sample is obtained, the hospital will conduct a preliminary examination and analysis to evaluate the sperm vitality and quantity. After that, one of two sperm storage methods can be selected, namely, cryopreservation or cryopreservation and identification.
Cryopreservation refers to the cryopreservation of sperm in liquid nitrogen to maintain its vitality. This method can keep the fertilization ability of sperm for a long time and can be used for multiple IVF cycles. Cryopreservation and identification is to check the quality of sperm before cryopreservation, and select the sperm most likely to be successfully fertilized for preservation by evaluating the morphology and motility of sperm.
Preliminary follow-up treatment
After obtaining appropriate sperm samples, the hospital will conduct preliminary treatment for them to prepare for the next step of IVF. Treatment at this stage usually includes sperm washing and concentration to remove other ingredients and increase sperm concentration.
During the washing process, the hospital uses special washing solution and culture medium to clean and purify the sperm samples, and remove the inactive and abnormal sperm. Next, the washed semen is centrifuged to concentrate the sperm sample, improve its fertilization ability and reduce the impact of potential host cells on sperm.
Summary
The success of IVF is closely related to the quality of sperm. The selection of donors, the method and process of sperm collection, as well as sperm storage and subsequent processing are all important steps to realize IVF. With the progress of modern medical technology, more and more couples are able to realize their reproductive dreams through IVF technology.
