Techniques for IVF application
IVF is a method of fertilizing and cultivating embryos in vitro through assisted reproductive technology, and then transferring the embryos back to the mother, which has helped countless couples achieve their reproductive aspirations worldwide. The application of IVF technology is inseparable from a series of sophisticated technical means and equipment support. The following will introduce some applied technologies from four aspects of IVF.
Egg collection technology
Egg collection is one of the key steps in IVF technology. Usually, egg collection will be carried out after the woman's ovulation period is monitored. First, doctors will use ultrasound to observe the development of follicles in the ovary. After confirming the maturity of follicles, they can collect eggs. The technique of vaginal needle insertion is generally used for egg collection. The doctor uses a slender needle to enter the ovary through the vagina and suck out the mature eggs.
In order to improve the maturity of eggs and increase the success rate of collection, doctors may also use follicle stimulating agents. By injecting women with specific hormones, the ovaries can produce more follicles, thus providing more eggs for collection.
In the actual operation process, doctors need to use a microscope to locate and extract eggs. This requires superb technology and experience to ensure the safety and success of the entire process.
Sperm processing technology
In IVF technology, sperm processing is another important link. First, before sperm collection, doctors may ask men to avoid ejaculation within a few days to improve the quality and concentration of sperm. Then, the collected sperm is processed and screened to obtain the best quality sperm.
The methods of sperm treatment mainly include centrifugation, osmosis and special culture medium. Among them, centrifugation is to put semen into a centrifuge tube for centrifugation to separate the most active and healthy sperm. The osmotic rule is to screen the sperm through specific osmotic solution according to the vitality and morphological characteristics of the sperm. The special culture medium rule is to provide a suitable culture environment to enhance the survival rate of sperm.
The application of these treatment methods can improve the vitality and quality of sperm and the success rate of fertilization.
Embryo culture technology
Embryo culture is another important step in IVF technology. After the egg is fertilized, the embryo will enter the culture dish for culture. In this process, doctors need to use special culture media and culture dishes to provide suitable environment and nutrients for embryo growth and development.
At present, the commonly used embryo culture techniques include traditional culture method and incubator culture method. The traditional culture method is to put the fertilized egg into the culture dish and place it in the incubator, and regularly check the embryonic development. Incubator culture principle is to use incubator equipment to provide constant temperature, humidity and carbon dioxide concentration, so as to realize the whole process of dry pre culture.
The application of embryo culture technology can improve the development quality and survival rate of embryos, and further improve the success rate of IVF.
Embryo transfer technology
Embryo transfer is the last and most critical step of IVF technology. Embryo transfer needs to accurately place the cultured embryos into the mother's uterus to achieve pregnancy. In the process of embryo transfer, doctors need to use microscopes and special transfer needles to complete.
Embryo transfer technology has high difficulty and risk, and the success rate is closely related to doctors' experience and technology. Some hospitals and specialized institutions have invested a lot of money and human resources in embryo transfer technology, and adopted advanced equipment and technology, which has improved the success rate of embryo transfer.
Summary
The application of IVF technology involves egg collection, sperm processing, embryo culture and embryo transfer. Through egg collection technology, mature eggs can be obtained for fertilization; Sperm treatment technology improves the success rate of fertilization; Embryo culture technology improves the quality of embryo development; Embryo transfer technology successfully places the cultured embryos into the mother's uterus. The application and development of these technologies have provided hope and opportunities for many infertile couples and promoted the rapid development of IVF technology.
