Operation steps and precautions of IVF
IVF is a method to help couples who cannot conceive naturally to achieve their reproductive aspirations with the help of assisted reproductive technology. The following will elaborate on the operation steps and precautions of IVF from four aspects.

1. Preliminary assessment and preparation
First of all, the couple should go to the hospital for treatment. It is very important to choose a formal and professional reproductive medical center, such as the reproductive center of a hospital. The doctor will make a preliminary assessment to understand the couple's fertility and physical condition and determine whether it is suitable for IVF.
After the evaluation, the doctor will carry out a series of examinations on the patient, including blood biochemical examination, fertility examination, uterine and fallopian tube patency examination, etc. In addition, doctors may also carry out genetic tests to assess the risk of patients with genetic diseases.
Preparations include:
(1) Understand and sign the informed consent form to clarify the risks and possible complications of IVF.
(2) Receive training on assisted reproductive technology, and master the methods and precautions of self injection drugs.
(3) According to the doctor's requirements, adjust the living habits and diet to maintain a healthy body shape and living condition.
2. Ovulation promotion and egg retrieval
The next step is to promote ovulation and take eggs.
(1) Doctors usually give ovulation promoting drugs to promote the development of follicles to increase the number of eggs taken. The ovulation induction cycle usually lasts 10-14 days.
(2) After the follicle matures, the doctor will perform an egg retrieval operation. This operation usually uses local anesthesia, and the eggs are taken out through vaginal puncture.
(3) The removed eggs will be immediately sent to the laboratory for culture.
3. Fertilization and transplantation
Fertilization and embryo culture are carried out in the laboratory.
(1) The extracted eggs are fertilized with sperm in vitro to generate embryos.
(2) Embryos are cultured in the laboratory, usually until the second or third day.
(3) According to the doctor's advice, a single embryo or multiple embryos will be selected and transplanted into the mother's uterus. This process is usually carried out through thin layer puncture of endometrium.
4. Follow up observation and pregnancy detection
After transplantation, follow-up observation and pregnancy test are required.
(1) The serum β - HCG was examined 10-14 days after embryo transfer to determine whether pregnancy occurred.
(2) If the result of serum β - HCG is positive, it indicates that the pregnancy is successful, and multiple monitoring of progesterone and estrogen is required to maintain the stability of pregnancy.
(3) After 10 weeks of pregnancy, they can be transferred to the obstetrics and gynecology department to continue pregnancy management.
summary
Through the above four steps, the IVF operation can be completed. First, preliminary assessment and preparation are carried out to determine the conditions suitable for IVF. Then, ovulation induction and egg retrieval are carried out, eggs and sperm are fertilized in vitro and embryo culture is carried out. Finally, appropriate embryos were selected and transferred to the mother's uterus for follow-up observation and pregnancy detection. Through scientific operation and patient cooperation, IVF technology has solved the problem of fertility for couples who cannot conceive naturally, and realized their fertility aspirations.