Which inspections are conducive to ensuring the quality of embryo development after the IVF is filed
In vitro fertilization (IVF) means that sperm and eggs are combined together to cultivate and conceive embryos, and high-quality embryos are transferred into the mother's body, and finally pregnancy and childbirth are achieved. In the process of IVF diagnosis and treatment, the doctor will establish a personal baby file for each patient, and the doctor will carry out multiple checks according to the specific situation of the patient to ensure the quality of embryo development. The following will elaborate on the inspection items that are conducive to ensuring the quality of embryo development after the establishment of IVF files from four aspects: embryo quality inspection, genetic inspection, immunological inspection and semen quality inspection.
Embryo quality inspection
Embryo quality is critical to the success rate of IVF. After filing, doctors will conduct embryo quality inspection to assess the health of embryos, including the number of embryonic cells, cell morphology, blastocyst formation, etc. These tests can help doctors select the most dynamic and potential embryos for transplantation, and increase the success rate of pregnancy.
1. Examination of embryonic cell number: through microscope observation, judge whether the number of embryonic cells is normal, usually 8-10 cells have good embryonic quality.
2. Cell morphology inspection: observe whether the embryonic cell morphology is normal, whether the cell division is even, and whether there is any abnormality. If there is abnormal cell morphology, it may indicate that the embryo has chromosome abnormalities.
3. Blastocyst formation inspection: observe the development of embryos in the culture process to see if they can develop into blastocysts successfully. Embryos with high blastocyst formation usually develop well.
Genetic examination
Genetic examination in IVF archiving is mainly to exclude or reduce the risk of genetic diseases carried by embryos. Genetic tests can help doctors screen embryos for chromosomal abnormalities and monogenic genetic diseases.
1. Chromosome karyotype inspection: use chromosome analysis technology to check the karyotype of embryos to determine whether the chromosomes are normal. Chromosome abnormalities may lead to abnormal embryonic development or pregnancy failure.
2. Single gene genetic disease screening: evaluate the family genetic disease history of the patient, and screen whether the embryo carries the pathogenic gene through genetic examination.
Immunological examination
Immunological examination can understand the immune status of patients, including the detection of immune related indicators, which can affect the success rate of IVF.
1. Natural killer cell activity test: to understand the immune function of patients by detecting the activity of natural killer cells. Abnormal immune function may lead to fertility problems or difficulties in IVF.
2. Immune factor test: detect the level of immune factors in the patient's blood, evaluate the status of the immune system, and understand whether it will have a negative impact on the IVF process.
Semen quality inspection
Semen quality is also critical to the success rate of IVF. After filing, the doctor will check the quality of the man's semen to ensure that the quality of sperm meets the requirements of fertilization.
1. Sperm concentration inspection: determine the concentration of sperm in semen by microscopic observation and calculation. The concentration of sperm in normal semen should be within the normal range.
2. Sperm vitality inspection: observe the sperm vitality, mobility and speed. Sperm with high vitality is of great significance for fertilization and embryonic development.
3. Sperm morphology inspection: check whether the sperm morphology is normal, and exclude the influence of abnormal morphology that may lead to difficulty in fertilization.
To sum up, the inspection items of IVF after filing include embryo quality inspection, genetics inspection, immunology inspection and semen quality inspection. These examinations help doctors understand the development of embryos and the health of patients, so as to select the most suitable treatment scheme and improve the success rate of IVF.
