Screening methods and standards of test tube baby test items
The screening methods and standards of IVF test items are to ensure the health of embryos and improve the success rate of pregnancy. The screening methods and standards of IVF test items are introduced in detail from four aspects: embryo quality, genetic disease screening, chromosome abnormality screening and hormone level detection.
1. Embryo quality
Embryo quality is critical to the development and success of IVF. In the IVF inspection project, the embryo quality is usually evaluated by the following indicators:
First, the embryonic development stage. Generally speaking, embryos are divided into different development stages from fertilization to transfer, including fertilized egg, two cell stage, four cell stage, eight cell stage, etc. The successful rate of pregnancy can be improved by selecting normal embryos for transplantation.
Second, the number and shape of cells. The number of normally developed embryonic cells should conform to the corresponding growth curve, and the morphology of embryonic cells should be normal without obvious abnormality.
Third, embryonic endoplasm. The embryonic endoplasm includes the absorption of embryonic nutrients and the integrity of embryonic cell membrane. A good embryonic endoplasm contributes to the growth and development of the embryo.
2. Genetic disease screening
Genetic disease is one of the important factors for the success of IVF. In the IVF test items, the common screening methods for genetic diseases include:
First, family history and personal medical history investigation. Understanding the genetic disease history of both parties and their families can help doctors assess the risk of embryonic genetic diseases.
Secondly, genetic counseling and gene testing. Through genetic counseling and gene testing, the genes of both sides can be analyzed to determine whether there is genetic disease related gene mutation. Embryos with a certain risk of genetic disease can be selectively transferred.
Finally, embryo biopsy and genetic analysis. In families with certain genetic diseases, embryo biopsy and genetic analysis can be carried out to screen embryos without genetic disease mutations for transplantation, so as to avoid the inheritance of genetic diseases.
3. Chromosome abnormality screening
Chromosome abnormality is a major factor in the failure of IVF, and it is also a common problem in the process of embryo rearing. Common screening methods for chromosomal abnormalities in IVF examination items include:
First, PGS (embryonic chromosome screening) technology. PGS technology can screen embryos with abnormal chromosome number and structure through genetic analysis of embryonic cells, so as to improve the success rate of pregnancy.
Secondly, NIPT (non-invasive prenatal gene detection) technology. NIPT technology is non-invasive and highly accurate in screening embryo chromosome abnormalities (such as Down's syndrome, Edward's syndrome, etc.) through embryonic DNA fragments in maternal blood.
Finally, embryo nuclear bias detection. Embryonic nuclear bias detection can screen embryos with abnormal chromosomes by observing the location and morphology of embryonic nuclei.
4. Hormone level detection
The regulation of hormone levels is critical to embryonic health and pregnancy success. In the test tube baby test items, the routine hormone level tests include:
First, serum hormone detection. Through monitoring the hormone level in women's blood, we can know whether women's ovarian function and hormone level are normal.
Secondly, endometrial thickness was measured. The thickness of endometrium is checked by B-ultrasound to determine whether it is suitable for IVF transplantation and determine the best transplantation time.
Finally, luteal function test. By monitoring the luteal hormone level in luteal phase, we can judge whether the luteal function is normal, so as to regulate the hormone level and promote the smooth progress of implantation and pregnancy.
summary
The screening methods and standards of IVF test items mainly include embryo quality, genetic disease screening, chromosome abnormality screening and hormone level detection. The evaluation of embryo quality, screening of genetic diseases, detection of chromosomal abnormalities and monitoring of hormone levels can improve the pregnancy success rate of IVF and reduce the risk of embryo abnormalities and genetic diseases. The screening methods and standards of IVF test items are of great significance to ensure the healthy development of IVF and the safety of mothers and infants.
