Key steps of the third generation IVF
The third-generation IVF technology is an important breakthrough in assisted reproductive technology, which enables couples who cannot conceive naturally to achieve their reproductive aspirations. It realizes pregnancy by fertilizing eggs and sperm in vitro, and then implanting the fertilized eggs into the mother. The key steps of the third generation IVF will be described in detail below.

Egg collection and sperm collection
The first step of three generations of IVF is egg collection and sperm collection. Egg collection is usually carried out in the gynecology and obstetrics hospital where the woman's menstrual period is coming. Doctors will use ultrasound-guided puncture technology to draw mature eggs from a slender aspirator into the ovary through the vagina. Sperm collection is the responsibility of the man, and can be collected by manual sperm discharge, machine brushing sperm discharge and other technologies.
After obtaining eggs and sperm, doctors will store them separately to prevent confusion or cross fertilization.
In vitro fertilization and embryo culture
In vitro fertilization is one of the key steps of the third generation IVF. In the process of in vitro fertilization, the doctor places the egg and sperm in the culture dish, and simulates the conditions in the human body through the culture medium to help the sperm combine with the egg and fertilize. The fertilization process generally requires 24 hours of observation to determine whether it is successful.
If fertilization is successful, the embryo will continue to grow and develop in the culture dish. Doctors regularly check the quality and development of embryos to determine the best time for transfer.
embryo transfer
In the process of embryo culture, doctors will choose the best transfer time according to the quality and development of the embryo. There are generally two methods of transplantation: intrauterine transplantation and extrauterine transplantation.
Intrauterine transplantation is the most common method. Doctors use a slender catheter to put the best quality embryos into the woman's uterus. Extrauterine transplantation is to place the embryo in other parts of the woman's uterus, such as the cervix or myometrium. This method is suitable for special cases, such as uterine malformations.
After transplantation, the doctor will observe the pregnancy of women and carry out necessary auxiliary drug treatment to promote embryo implantation and pregnancy development.
Pregnancy monitoring and delivery
After implantation of successfully transplanted embryos, doctors will conduct pregnancy monitoring, including blood tests and ultrasound tests. These tests can help doctors determine whether the pregnancy is normal and whether the embryo has successfully developed.
If the pregnancy develops normally, the couple will carry out pregnancy care and prenatal examination according to the process of normal pregnancy. When the birth process comes, the woman will deliver according to the normal delivery procedure, so as to realize the birth of three generations of test tube babies.
summary
The key steps of the third generation IVF include egg collection and sperm collection, in vitro fertilization and embryo culture, embryo transfer, and pregnancy monitoring and delivery. Each step requires highly professional medical technology and rigorous operation to ensure success rate and safety. Three generations of IVF technology provides hope for couples who cannot conceive naturally, and it is also one of the great achievements of modern medicine.