The way of semen extraction in IVF
IVF technology is a kind of assisted reproductive technology. It takes out male semen and female eggs, fertilizes them in vitro in the laboratory, and then transfers the fertilized eggs back to the female uterus to achieve the purpose of pregnancy and fertility. In the process of IVF, sperm extraction is a crucial step. The following will elaborate on the semen extraction methods of IVF from four aspects: semen collection, semen extraction techniques, semen processing and semen preservation.
1. Semen collection
Semen collection is the first step in IVF. Generally, men will be required to collect semen in specific rooms or rooms of professional hospitals. There are several semen collection methods as follows:
The first is masturbation collection method, in which men masturbate themselves in a specific environment, and then collect the collected semen into a cup.
The second method is sex collection, that is, having sexual intercourse with a partner, and then collecting semen into a cup while the partner ejaculates outside the body.
Drug stimulation is also a commonly used method of semen collection. Doctors may, according to the specific circumstances, use drugs for men or promote semen excretion through electrical stimulation.
2. Essence extraction technique
Sperm extraction means that after semen collection, it is concentrated to extract the sperm contained in ejaculation. Common methods of extracting semen are as follows:
The first method is centrifugation. The collected semen is put into a centrifuge, and the semen with different components is stratified by the high-speed rotation of centrifugation, and then the layer containing high concentration sperm is extracted.
The second is osmotic method. By putting the collected semen into a culture dish containing nutrient solution, the sperm with high vitality can swim to the liquid, while the unhealthy sperm or the sperm with low vitality can stay in place.
In addition, there is a magnetic separation method, in which magnetic particles are added to semen, and magnetic fields are used to gather the particles rich in healthy sperm together, and then separate them.
3. Treatment of semen
The purpose of semen treatment is to remove the blood, mucus and other inappropriate components around the sperm, and improve the semen quality and pregnancy success rate. Specific semen treatment methods include:
First, semen is diluted with special solution to separate high-quality sperm.
Then, the diluted semen was stratified by centrifugation, and the layer rich in highly active sperm was extracted.
Finally, through the use of filter paper filtration and other methods, the impurities and inappropriate ingredients in semen can be removed to make semen more pure.
4. Preserve semen
The purpose of semen preservation is to be free from the limitation of men's physical conditions when taking semen, so that it can be supplied to patients in need at any time. At present, there are two common ways to preserve semen:
One is cryopreservation of semen. The semen collected will be cryopreserved with special freezing liquid. This method can preserve sperm for a long time, but it will lose some vitality after thawing.
The other is liquid nitrogen cryopreservation of semen. The collected semen is cryopreserved in liquid nitrogen, which can better protect the vitality and quality of sperm, and save for a longer time.
Summary
The methods of semen extraction for IVF include semen collection, semen extraction techniques, semen processing and semen preservation. Semen can be collected by means of masturbation, sex and drug stimulation. High quality sperm can be extracted by centrifugation, osmosis and magnetic separation. The semen can be treated by dilution, centrifugation and filtration to remove impurities and improve the quality of semen. Cryopreservation or liquid nitrogen cryopreservation can be selected for semen preservation, so that it can be supplied to patients in need at any time. The application of these methods has enabled the successful implementation of IVF technology and helped couples unable to conceive naturally realize their dreams of life.
