Steps of the second test tube baby
Two child test tube baby refers to the assisted reproductive technology used in a family with one child to get pregnant again. This process includes the following steps:

1. Preliminary assessment and preparation
First, both husband and wife need to carry out physical examination, including excluding the possibility of abnormal structure and function of sexual reproductive organs, to ensure that the wife's ovary functions normally and has the potential to supply enough eggs. Doctors may also require couples to undergo a series of blood and genetic tests to assess the risk of suffering from any genetic defect or genetic disease.
After completing the physical assessment, the doctor will have a detailed understanding of the couple's reproductive history and lifestyle to understand the possible infertility factors, such as age, life pressure, habits, etc. This will also help doctors determine which IVF technology is suitable for couples next.
Finally, couples need to provide relevant documents, such as ID cards, marriage certificates and birth certificates of existing children.
2. Ovarian stimulation and egg collection
In the ovarian stimulation phase, the wife will receive a series of drugs to stimulate the ovary to produce more follicles. Doctors will regularly monitor the growth of follicles, usually through ultrasound and blood tests to determine the best time to pick.
Once the follicles have developed to an appropriate stage, doctors will use transvaginal ultrasound probes and puncture needles in the operating room to collect mature eggs. This operation is usually performed in a short time under local anesthesia.
After the eggs are collected, the doctor will store them in the in vitro culture medium and fertilize them with the husband's sperm in vitro soon.
3. In vitro fertilization and embryo culture
At the stage of in vitro fertilization, the collected eggs and the husband's sperm are placed in a culture dish and cultured and observed under appropriate conditions. If the sperm quality is low, doctors may choose to directly inject single sperm into the egg, a process known as single sperm injection (ICSI).
During the culture process, the doctor will closely monitor the development of the fertilized egg, which usually lasts for 5 to 6 days until it develops into a blastocyst. Then the doctor will select the best embryo for implantation.
4. Embryo implantation
In the embryo implantation stage, doctors will select the most suitable embryo and put it into the woman's uterus to help embryo implantation and development. Anesthesia is not required for this procedure, but doctors may use ultrasound guidance to ensure accurate implantation.
After implantation, the patient needs to observe for a period of time and conduct blood pregnancy test regularly to detect whether the pregnancy is successful.
summary
The steps of IVF include preliminary evaluation and preparation, ovarian stimulation and egg collection, in vitro fertilization and embryo culture, and embryo implantation. Through these steps, couples can increase their chances of getting pregnant again and realize their dreams of family.