IVF is an assisted reproductive technology that helps couples who cannot conceive naturally realize their reproductive dreams through in vitro fertilization and embryo transfer.The whole process needs multiple steps and cycles to complete.This article will elaborate the process and steps of IVF from four aspects.
1. Diagnosis and evaluation
The first step is to carry out diagnosis and evaluation to determine whether it is suitable for IVF.Couples need a comprehensive physical examination, including semen analysis, endocrine hormone testing, ovarian function evaluation, etc.At the same time, women need to conduct tubal patency inspection to ensure a good fertilization environment.Doctors will also learn about the couple's medical history, including infertility causes, genetic diseases, etc.According to the evaluation results, the doctor will inform the couple whether it is suitable for IVF and develop a personalized treatment plan.The next stage is hormone therapy.Women will receive ovulation induction treatment according to their individual conditions.Gonadotropin is injected to stimulate ovarian development and increase the number of follicles.Doctors will regularly monitor the growth of follicles, and determine the best time for egg retrieval through ultrasonic examination and blood hormone detection.
2. Egg taking and semen treatment
When the follicles are fully developed, the egg retrieval process begins.This needs to be done in the operating room.The doctor will puncture the ovary with a fine needle to extract mature eggs under the guidance of colposcopy or B-ultrasound.This process usually does not require general anesthesia, but only local anesthesia.After taking the eggs, they will be sent to the laboratory for treatment immediately.At the same time, the man collected semen samples.The semen sample will be centrifuged to separate active and healthy sperm.
3. Fertilization and embryo culture
In the laboratory, eggs and sperm are fertilized in vitro.Common methods include "standard fertilization" and "microinjection fertilization".The standard fertilization is to put the egg and sperm in the same culture dish for natural fertilization, while microinjection fertilization is to inject a sperm into the egg through microscopic technology.The fertilized egg will continue to grow in the culture dish and form an embryo.The embryo culture generally lasts for 2-6 days, during which the doctor will judge whether it is suitable for embryo transfer according to the quality and development of the embryo.
4. Embryo transfer and follow-up
Embryo transfer is the last step in the IVF process.High quality and properly developed embryos were selected and placed into the uterus through catheters.This process usually does not require general anesthesia and can be completed in the outpatient department of the hospital.After transplantation, women need to continue taking progesterone and other drugs to help maintain embryo implantation and pregnancy development.About 14 days later, blood progesterone test was performed to confirm whether or not she was pregnant.If the test result is positive, the doctor will continue to conduct pregnancy monitoring and care until the pregnancy is stable.
summary
IVF is a complex process that requires many steps and cycles.The diagnosis and evaluation stage determines the patient's indications, the hormone treatment stage promotes ovulation, the oocyte collection and semen treatment to obtain eggs and sperm, the fertilization and embryo culture stage generates and cultivates embryos, and embryo transfer and subsequent stages help embryo implantation and development.Each stage requires the careful cooperation and continuous attention of doctors and patients.Through IVF technology, many couples have realized their reproductive dreams.
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